Fig 1: MK8722 disrupts autophagic vesicle-lysosome fusion and inhibits SNARE complexes formation. (A, B) A2780 and OV90 cells were cultured in MK (20 μM, 40 μM) or an equal volume of DMEM medium for 48 h, and the expression of autophagy upstream-related proteins (PI3K, AKT, mTOR, p-PI3K, p-AKT, and p-mTOR) was analyzed by western blotting. GAPDH was used as a loading control. Quantification and analysis of western blotting results (p-mTOR, p-PI3K, and p-AKT) were performed. (C) After transient transduction of EGFP-LC3, A2780 and OV90 cells were treated with MK (20 μM) for 48 h. Fluorescence signals were examined under confocal microscopy after staining with LysoTracker Red. Scale bar: 10 μm. (D, F) Representative images showing STX17 (green) and VAMP8 (red) of A2780 and OV90 cells after 48 h of treatment with MK (20 μM). (E, G) Quantitative analysis of the relationship between STX17 and VAMP8 in (C) and (D). Scale bar: 10 μm. Scatter plots were generated using Image Pro Plus software. Results are presented as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001. The results represent the mean values from three independent experiments (n = 3), and representative pictures are shown.